Three-dimensional mapping of intact ovaries reveals the aging dynamics of the ovarian reserve

Arturo D'Angelo, Daniel Franco-Barranco, Elvan Boke

1. 01

   Over 85,000 mouse oocytes mapped in intact ovaries using whole-organ imaging, AI segmentation and modelling

2. 02

   The fraction of newly activated oocytes stayed stable across aging even as the reserve collapsed

3. 03

   Implies organ-wide control of oocyte activation rather than a fixed per-oocyte activation rate

4. 04

   Challenges the simple burn-rate model of ovarian reserve depletion

Study Design

Preclinical Study

Sample Size

Not reported

Study Duration

Not reported

Methodology

Whole-ovary light-sheet-class imaging of intact murine ovaries with AI segmentation and mathematical modelling of activation dynamics across age.

Limitations

Murine only. Whether the same organ-level feedback control of oocyte activation operates in human ovaries is untested, so this does not directly change AMH interpretation or fertility counselling in people.